Predicting Cell Stress and Strain during Extrusion Bioprinting
Müller SJ, Fabry B, Gekle S (2023)
Publication Type: Journal article
Publication year: 2023
Journal
Book Volume: 19
Article Number: 064061
Journal Issue: 6
DOI: 10.1103/PhysRevApplied.19.064061
Abstract
Bioprinting of living cells can cause major shape deformations, which may severely affect cell survival and functionality. While the shear stresses occurring during cell flow through the printer nozzle have been quantified to some extent, the extensional stresses occurring as cells leave the nozzle into the free printing strand have been mostly ignored. Here we use lattice Boltzmann simulations together with a finite-element based cell model to study cell deformation inside the nozzle and at its exit. Our simulation results are in good qualitative agreement with experimental microscopy images. We show that, for cells flowing in the center of the nozzle, extensional stresses can be significant while, for cells flowing off-center, their deformation is dominated by the shear flow inside the nozzle. From the results of these simulations, we develop two simple methods that only require the printing parameters (nozzle diameter, flow rate, bioink rheology) to (i) accurately predict the maximum cell stress occurring during the three-dimensional bioprinting process and (ii) approximately predict the cell strains caused by the elongational flow at the nozzle exit.
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Germany (DE)How to cite
APA:
Müller, S.J., Fabry, B., & Gekle, S. (2023). Predicting Cell Stress and Strain during Extrusion Bioprinting. Physical Review Applied, 19(6). https://doi.org/10.1103/PhysRevApplied.19.064061
MLA:
Müller, Sebastian J., Ben Fabry, and Stephan Gekle. "Predicting Cell Stress and Strain during Extrusion Bioprinting." Physical Review Applied 19.6 (2023).
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