Simple, Economic, and Robust Rail-Based Setup for Super-Resolution Localization Microscopy

Almahayni K, Almahayni K, Nestola G, Spiekermann M, Möckl L (2023)

Publication Type: Journal article

Publication year: 2023

Journal

Journal of Physical Chemistry A American Chemical Society

DOI: 10.1021/acs.jpca.3c01351

Abstract

Research during the past 2 decades has showcased the power of single-molecule localization microscopy (SMLM) as a tool for exploring the nanoworld. However, SMLM systems are typically available in specialized laboratories and imaging facilities, owing to their expensiveness as well as complex assembly and alignment procedure. Here, we lay out the blueprint of a sturdy, rail-based, cost-efficient, multicolor SMLM setup that is easy to construct and align in service of simplifying the accessibility of SMLM. We characterize the optical properties of the design and assess its capabilities, robustness, and stability. The performance of the system is assayed using super-resolution imaging of biological samples. We believe that this design will make SMLM more affordable and broaden its availability.

Authors with CRIS profile

Karim Almahayni
Leonhard Möckl

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light DE Germany (DE)

How to cite

APA:

Almahayni, K., Almahayni, K., Nestola, G., Spiekermann, M., & Möckl, L. (2023). Simple, Economic, and Robust Rail-Based Setup for Super-Resolution Localization Microscopy. Journal of Physical Chemistry A. https://doi.org/10.1021/acs.jpca.3c01351

MLA:

Almahayni, Karim, et al. "Simple, Economic, and Robust Rail-Based Setup for Super-Resolution Localization Microscopy." Journal of Physical Chemistry A (2023).

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