Pfister F, Alexiou C, Janko C (2023)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2023
Publisher: Humana Press Inc.
Series: Methods in Molecular Biology
Book Volume: 2644
Pages Range: 331-346
DOI: 10.1007/978-1-0716-3052-5_21
Immune cell therapies, such as adoptive T cell therapies, are an innovative and powerful treatment option for previously non-treatable diseases. Although immune cell therapies are thought to be very specific, there is still the danger of developing severe to life-threatening side effects due to the unspecific distribution of the cells throughout the body (on-target/off-tumor effects). A possible solution for the reduction of these side effects and the improvement of tumor infiltration is the specific targeting of the effector cells (e.g., T cells) to the desired destination (e.g., tumor region). This can be achieved by the magnetization of cells with superparamagnetic iron oxide nanoparticles (SPIONs) for spatial guidance via external magnetic fields. A prerequisite for the use of SPION-loaded T cells in adoptive T cell therapies is that cell viability and functionality after nanoparticle loading are preserved. Here, we demonstrate a protocol to analyze cell viability and functionality such as activation, proliferation, cytokine release, and differentiation at a single cell level using flow cytometry.
APA:
Pfister, F., Alexiou, C., & Janko, C. (2023). Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields. In (pp. 331-346). Humana Press Inc..
MLA:
Pfister, Felix, Christoph Alexiou, and Christina Janko. "Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields." Humana Press Inc., 2023. 331-346.
BibTeX: Download