Abdelrahman A, Smith AS, Sengupta K (2023)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2023
Publisher: Humana Press Inc.
Series: Methods in Molecular Biology
Book Volume: 2654
Pages Range: 123-135
DOI: 10.1007/978-1-0716-3135-5_8
Reflection interference contrast microscopy (RICM) is an optical microscopy technique ideally suited for imaging adhesion. While RICM (and the closely related interference reflection microscopy (IRM)) has been extensively used qualitatively or semiquantitatively to image cells, including immune cells, it can also be used quantitatively to measure membrane to surface distance, especially for model membranes. Here, we present a protocol for RICM and IRM imaging and the details of semiquantitative and quantitative analysis.
APA:
Abdelrahman, A., Smith, A.-S., & Sengupta, K. (2023). Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy. In (pp. 123-135). Humana Press Inc..
MLA:
Abdelrahman, Ahmed, Ana-Suncana Smith, and Kheya Sengupta. "Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy." Humana Press Inc., 2023. 123-135.
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