Friedrich B, Vogel P, Rückert MA, Lyer S, Günther J, Wernery U, Joseph S, Müller J, Behr VC, Alexiou C, Tietze R (2023)
Publication Type: Journal article
Publication year: 2023
DOI: 10.1007/s00253-023-12513-4
Abstract: Pandemics like SARS-Cov-2 very frequently have their origin in different animals and in particular herds of camels could be a source of zoonotic diseases. This study took advantage on a highly sensitive and adaptable method for the fast and reliable detection of viral antibodies in camels using low-cost equipment. Magnetic nanoparticles (MNP) have high variability in their functionalization with different peptides and proteins. We confirm that 3-aminopropyl triethoxysilane (APTES)-coated MNP could be functionalized with viral proteins. The protein loading could be confirmed by simple loading controls using FACS-analysis (p < 0.05). Complementary combination of antigen and antibody yields in a significant signal increase could be proven by both FACS and COMPASS. However, COMPASS needs only a few seconds for the measurement. In COMPASS, the phase φ
APA:
Friedrich, B., Vogel, P., Rückert, M.A., Lyer, S., Günther, J., Wernery, U.,... Tietze, R. (2023). Detection of viral antibodies in camel sera using magnetic particle spectroscopy. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-023-12513-4
MLA:
Friedrich, Bernhard, et al. "Detection of viral antibodies in camel sera using magnetic particle spectroscopy." Applied Microbiology and Biotechnology (2023).
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