A specific spectral signature of serum and plasma-derived extracellular vesicles for cancer screening

Krafft C, Wilhelm K, Eremin A, Nestel S, Von Bubnoff N, Schultze-Seemann W, Popp J, Nazarenko I (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Nanomedicine-Nanotechnology Biology and Medicine Elsevier

Book Volume: 13

Pages Range: 835-841

Journal Issue: 3

DOI: 10.1016/j.nano.2016.11.016

Abstract

In cancer, extracellular vesicles (EV) contribute to tumor progression by regulating local and systemic effects. Being released into body fluids, EV may be used in nanomedicine as a valuable source for diagnostic biomarkers. In this work, infrared and Raman spectroscopy were used for comprehensive comparative analysis of cancer versus non-cancer EV and patient screening. Two different EV fractions enriched in exosomes and microvesicles were isolated by differential centrifugation from serum and plasma of cancer and non-cancer patients and from serum and plasma of a healthy donor. The EV fractions were then subjected to drop-coating deposition and drying on calcium fluoride substrates. Reduction of alpha-helix-rich proteins and enhancement of beta-sheet-rich proteins as a cancer-specific blood EV signature was determined, and subsequently this feature was applied for a pilot study aiming to detect prostate cancer in a test cohort of patients with high-grade prostate carcinoma and benign hypoplasia.

Involved external institutions

Albert-Ludwigs-Universität Freiburg DE Germany (DE) Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE)

How to cite

APA:

Krafft, C., Wilhelm, K., Eremin, A., Nestel, S., Von Bubnoff, N., Schultze-Seemann, W.,... Nazarenko, I. (2017). A specific spectral signature of serum and plasma-derived extracellular vesicles for cancer screening. Nanomedicine-Nanotechnology Biology and Medicine, 13(3), 835-841. https://doi.org/10.1016/j.nano.2016.11.016

MLA:

Krafft, Christoph, et al. "A specific spectral signature of serum and plasma-derived extracellular vesicles for cancer screening." Nanomedicine-Nanotechnology Biology and Medicine 13.3 (2017): 835-841.

BibTeX: Download