Confining Brownian motion of single nanoparticles in an ABELtrap
Dienerowitz M, Heitkamp T, Gottschall T, Limpert J, Boersch M (2017)
Publication Type: Conference contribution
Publication year: 2017
Journal
Publisher: SPIE
Book Volume: 10120
Conference Proceedings Title: Proceedings of SPIE - The International Society for Optical Engineering
Event location: San Francisco, CA, USA
ISBN: 9781510606814
DOI: 10.1117/12.2250550
Abstract
Trapping nanoscopic objects to observe their dynamic behaviour for extended periods of time is an ongoing quest. Particularly, sub-100nm transparent objects are hard to catch and most techniques rely on immobilisation or transient diffusion through a confocal laser focus. We present an Anti-Brownian ELectrokinetic trap1-7 (pioneered by A. E. Cohen and W. E. Moerner) to hold nanoparticles and individual FoF1-ATP synthase proteins in solution. We are interested in the conformational dynamics of this membrane-bound rotary motor protein that we monitor using single-molecule FRET. The ABELtrap is an active feedback system cancelling the nano-object's Brownian motion by applying an electric field. We show how the induced electrokinetic forces confine the motion of nanoparticles and proteoliposomes to the centre of the trap.
Involved external institutions
Germany (DE)How to cite
APA:
Dienerowitz, M., Heitkamp, T., Gottschall, T., Limpert, J., & Boersch, M. (2017). Confining Brownian motion of single nanoparticles in an ABELtrap. In Enrique J. Galvez, Jesper Gluckstad, David L. Andrews (Eds.), Proceedings of SPIE - The International Society for Optical Engineering. San Francisco, CA, USA: SPIE.
MLA:
Dienerowitz, Maria, et al. "Confining Brownian motion of single nanoparticles in an ABELtrap." Proceedings of the Complex Light and Optical Forces XI 2017, San Francisco, CA, USA Ed. Enrique J. Galvez, Jesper Gluckstad, David L. Andrews, SPIE, 2017.
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