Carbon nanodots based biosensors for gene mutation detection
Garcia-Mendiola T, Bravo I, Maria Lopez-Moreno J, Pariente F, Wannemacher R, Weber K, Popp J, Lorenzo E (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 256
Pages Range: 226-233
DOI: 10.1016/j.snb.2017.10.105
Abstract
An electrochemical DNA biosensor based on a carbon nanodots (CDs) modified screen-printed gold electrode as a transducer is reported in this work. CDs were synthesized by thermal carbonization of ethyleneglycol bis-(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) and characterized by different techniques (DLS, TEM, FTIR, Raman). The electrode surface modification was accomplished by drop-casting a suspension of CDs. SEM analysis and cyclic voltammetry were used to characterize the resulting modified electrode. Synthetic 25-mer or 100-mer DNA capture probes, capable to hybridize with a specific sequence of the pathogen Helicobacter pylori or the cystic fibrosis transmembrane regulator (CFTR) gene were attached to the CDs-gold surface. A 25-bases synthetic fully complementary sequence or a single nucleotide polymorphism to the DNA capture probe and a 373-bases PCR amplicon of exon 11 of CFTR containing a sequence complementary to the capture probe, were employed as target. The hybridization event was electrochemically monitored by using safranine as redox indicator, which selectively binds to double stranded DNA (dsDNA). A detection limit of 0.16 nM was obtained for the 25-mer synthetic target DNA. The biosensor shows a very high reproducibility and selectivity, allowing to detect a single nucleotide polymorphism. It has been applied to the detection of F508del mutation in the CFTR gene.
Involved external institutions
Universidad Autónoma de Madrid (UAM)
Spain (ES)
IMDEA-Nanociencia
Spain (ES)
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
Spain (ES)
IMDEA-Nanociencia
Spain (ES)
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
How to cite
APA:
Garcia-Mendiola, T., Bravo, I., Maria Lopez-Moreno, J., Pariente, F., Wannemacher, R., Weber, K.,... Lorenzo, E. (2018). Carbon nanodots based biosensors for gene mutation detection. Sensors and Actuators B-Chemical, 256, 226-233. https://dx.doi.org/10.1016/j.snb.2017.10.105
MLA:
Garcia-Mendiola, Tania, et al. "Carbon nanodots based biosensors for gene mutation detection." Sensors and Actuators B-Chemical 256 (2018): 226-233.
BibTeX: Download