Raman spectroscopic investigation of the human liver stem cell line HepaRG

Ryabchykov O, Braeutigam K, Galler K, Neugebauer U, Mosig A, Bocklitz T, Popp J (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Journal of Raman Spectroscopy Wiley-Blackwell

Book Volume: 49

Pages Range: 935-942

Journal Issue: 6

DOI: 10.1002/jrs.5392

Abstract

In this work, Raman spectroscopic cell imaging approaches and a discrimination between HepG2, nondifferentiated hepatic stem cell line HepaRG, and differentiated hepatocyte-like HepaRG cells are presented. Raman spectroscopic imaging was used to visualize the cell nuclei by means of false color imaging using a marker band, and a cell segmentation was performed by means of clustering. Furthermore, a 3-class-classification model based on the mean Raman spectra of individual cells was established for a classification between different cell types. A high average sensitivity of 96% was achieved by the applied classification model. Based on the results of clustering and classification, the main spectral contributions to different cell types and cell segments were analyzed in detail. Thereby, HepG2, nondifferentiated hepatic stem cell line HepaRG, and differentiated hepatocyte-like HepaRG cells were Raman spectroscopically characterized and proven to be significantly different.

Involved external institutions

Friedrich-Schiller-Universität Jena DE Germany (DE) Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) Universitätsklinikum Jena DE Germany (DE)

How to cite

APA:

Ryabchykov, O., Braeutigam, K., Galler, K., Neugebauer, U., Mosig, A., Bocklitz, T., & Popp, J. (2018). Raman spectroscopic investigation of the human liver stem cell line HepaRG. Journal of Raman Spectroscopy, 49(6), 935-942. https://doi.org/10.1002/jrs.5392

MLA:

Ryabchykov, Oleg, et al. "Raman spectroscopic investigation of the human liver stem cell line HepaRG." Journal of Raman Spectroscopy 49.6 (2018): 935-942.

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