One-step reduced/quinone functionalized graphene oxide as reagentless lactate biosensing platform

Bravo I, Revenga-Parra M, Weber K, Popp J, Pariente F, Lorenzo E (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Sensors and Actuators B-Chemical Elsevier

Book Volume: 267

Pages Range: 533-541

DOI: 10.1016/j.snb.2018.03.170

Abstract

One-step approach to simultaneously reduce and modify graphene oxide (GO) using N,N’-Bis(3,4-dihydroxybenzylidene)-1,2-diaminobenzene Schiff base ligand (3,4DHS), in order to obtain functionalized reduced graphene oxide (rGO-DHS), is described. Once the novel hybrid nanomaterial was characterized by spectroscopic and electrochemical techniques, confirming the presence of the characteristic quinone/hydroquinone moieties, it has been combined with lactate oxidase (LOx) to develop a sensitive and selective reagentless lactate biosensor. The resulting biosensing device shows a very good analytical performance with detection and determination limits of 2.9 and 9.7 μM, respectively. The RSD for three different devices was lower than 9%. The operational stability is more than one month. The applicability has been demonstrated by the direct determination of the L-lactate content in white wine.

Involved external institutions

Universidad Autónoma de Madrid (UAM) ES Spain (ES) Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE)

How to cite

APA:

Bravo, I., Revenga-Parra, M., Weber, K., Popp, J., Pariente, F., & Lorenzo, E. (2018). One-step reduced/quinone functionalized graphene oxide as reagentless lactate biosensing platform. Sensors and Actuators B-Chemical, 267, 533-541. https://dx.doi.org/10.1016/j.snb.2018.03.170

MLA:

Bravo, Iria, et al. "One-step reduced/quinone functionalized graphene oxide as reagentless lactate biosensing platform." Sensors and Actuators B-Chemical 267 (2018): 533-541.

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