Optical fiber based light scattering detection in microfluidic droplets
Wohlfeil S, Hengoju S, Munser AS, Tovar M, Shvydkiv O, Roth M, Schroeder S, Beckert E, Eberhardt R, Tuennerman A (2019)
Publication Type: Conference contribution
Publication year: 2019
Journal
Publisher: SPIE
Book Volume: 10875
Conference Proceedings Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Event location: San Francisco, CA, USA
ISBN: 9781510623927
DOI: 10.1117/12.2509248
Abstract
Droplet based microfluidic technology is a miniaturized platform for microbial analysis on picoliter scale. With its costefficiency, high-throughput and feasibility of complex handling protocols, droplet microfluidics is a favorable platform for applications such as microorganism screening or synthetic biology. Scattered-light-based microbial detection, in comparison to the widely used fluorescent-label-based approach, provides a contact-free and label-free, yet sensitive measuring solution. The angular dependency of scattered light delivers an elaborate information about the morphology and the physical properties, e.g. size and refractive index, of microbial samples. Due to the complexity and ambiguity of the droplet contents, an angle resolved scattered light detection system could provide powerful method for a label-free identification and quantification of the microbes in droplets. In this paper, a novel approach of light scattering measurement in Polydimethylsiloxane (PDMS) microfluidic chips is presented, engaging optical fibers for a light-scattering-based on-chip microbial detection. Optical fibers, with their fast readout and compact size, are very suitable for easier system integration towards flexible and versatile lab-on-a-chip applications.
Involved external institutions
Fraunhofer-Institut für Angewandte Optik und Feinmechanik (IOF) / Fraunhofer Institute for Applied Optics and Precision Engineering
Germany (DE)
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie (Hans-Knöll-Institut (HKI)) / Leibniz Institute for Natural Product Research and Infection Biology
Germany (DE)
Germany (DE)
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie (Hans-Knöll-Institut (HKI)) / Leibniz Institute for Natural Product Research and Infection Biology
Germany (DE)
How to cite
APA:
Wohlfeil, S., Hengoju, S., Munser, A.-S., Tovar, M., Shvydkiv, O., Roth, M.,... Tuennerman, A. (2019). Optical fiber based light scattering detection in microfluidic droplets. In Holger Becker, Bonnie L. Gray (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. San Francisco, CA, USA: SPIE.
MLA:
Wohlfeil, Shulin, et al. "Optical fiber based light scattering detection in microfluidic droplets." Proceedings of the Microfluidics, BioMEMS, and Medical Microsystems XVII 2019, San Francisco, CA, USA Ed. Holger Becker, Bonnie L. Gray, SPIE, 2019.
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