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Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology

Harberts J, Zierold R, Fendler C, Koitmaee A, Bayat P, Fernandez-Cuesta I, Loers G, Diercks BP, Fliegert R, Guse AH, Ronning C, Otnes G, Borgstrom MT, Blick RH (2019)

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Publication Type: Journal article

Publication year: 2019

Journal

Book Volume: 9

Pages Range: 11194-11201

Journal Issue: 20

DOI: 10.1039/c8ra05320k

Abstract

Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al2O3 coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.

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How to cite

APA:

Harberts, J., Zierold, R., Fendler, C., Koitmaee, A., Bayat, P., Fernandez-Cuesta, I.,... Blick, R.H. (2019). Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology. RSC Advances, 9(20), 11194-11201. https://doi.org/10.1039/c8ra05320k

MLA:

Harberts, Jann, et al. "Culturing and patch clamping of Jurkat T cells and neurons on Al2O3 coated nanowire arrays of altered morphology." RSC Advances 9.20 (2019): 11194-11201.

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