Muehlig A, Jahn IJ, Heidler J, Jahn M, Weber K, Sheen P, Zimic M, Cialla-May D, Popp J (2019)
Publication Type: Journal article
Publication year: 2019
Book Volume: 9
Article Number: 511
Journal Issue: 12
DOI: 10.3390/app9122511
The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 μm for PZA and 21 μm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA.
APA:
Muehlig, A., Jahn, I.J., Heidler, J., Jahn, M., Weber, K., Sheen, P.,... Popp, J. (2019). Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids. Applied Sciences, 9(12). https://dx.doi.org/10.3390/app9122511
MLA:
Muehlig, Anna, et al. "Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids." Applied Sciences 9.12 (2019).
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