Label-free CARS microscopy through a multimode fiber endoscope
Tragardh J, Pikalek T, Sery M, Meyer T, Popp J, Cizmar T (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 27
Pages Range: 30055-30066
Journal Issue: 21
DOI: 10.1364/OE.27.030055
Abstract
Multimode fibres have recently been employed as high-resolution ultra-thin endoscopes, capable of imaging biological structures deep inside tissue in vivo. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fibre endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial 125 µm diameter, 0.29 NA GRIN fibre, and wavefront shaping on an SLM is used to create foci that are scanned behind the fibre facet across the sample. The chemical selectivity is demonstrated by imaging 2 µm polystyrene and 2.5 µm PMMA beads with per pixel integration time as low as 1 ms for epi-detection.
Involved external institutions
Institute of Scientific Instruments of the Czech Academy of Sciences (ISI CAS) / Ústav Přístrojové Techniky (ÚPT AV ČR)
Czech Republic (CZ)
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
Czech Republic (CZ)
Leibniz-Institut für Photonische Technologien e.V.
Germany (DE)
How to cite
APA:
Tragardh, J., Pikalek, T., Sery, M., Meyer, T., Popp, J., & Cizmar, T. (2019). Label-free CARS microscopy through a multimode fiber endoscope. Optics Express, 27(21), 30055-30066. https://doi.org/10.1364/OE.27.030055
MLA:
Tragardh, Johanna, et al. "Label-free CARS microscopy through a multimode fiber endoscope." Optics Express 27.21 (2019): 30055-30066.
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