Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope

Tragardh J, Pikalek T, Sery M, Akimov D, Meyer T, Popp J, Cizmar T (2020)

Publication Type: Conference contribution

Publication year: 2020

Journal

Proceedings of SPIE Society of Photo-optical Instrumentation Engineers (SPIE)

Publisher: SPIE

Book Volume: 11359

Conference Proceedings Title: Proceedings of SPIE - The International Society for Optical Engineering

Event location: Virtual, Online, FRA

ISBN: 9781510634909

DOI: 10.1117/12.2555080

Abstract

Advanced wavefront-shaping methods can be used to transform a simple multimode fiber into an ultra-thin laser scanning microscope. Here, we extend this technique to label-free non-linear microscopy with chemical contrast using coherent anti-Stokes Raman scattering (CARS) through a multimode fiber endoscope, which opens up new avenues for instant and in-situ diagnosis of potentially malignant tissue. We use a commercial, 125 μm diameter, 0.29 NA, GRIN fiber as the endoscopic probe. Wavefront shaping on a spatial light modulator is used to create a focus, where the 1-2 ps long pump and Stokes pulses are overlapped in time, which is scanned behind the fiber facet across the sample. The chemical selectivity is demonstrated by imaging 2 μm polystyrene and 2.5 μm PMMA beads with per pixel integration time as low as 1 ms for epi-detection. Epi-detection through the fiber is possible despite the fact that the CARS signal is emitted mainly in the forward direction, away from the fiber facet. Detecting the back-scattered signal from the underlying tissue, requires a large detector aperture to be efficient. By detecting through both the core and the cladding of the fiber, we obtain sufficient detection efficiency.

Involved external institutions

Institute of Scientific Instruments of the Czech Academy of Sciences (ISI CAS) / Ústav Přístrojové Techniky (ÚPT AV ČR) CZ Czech Republic (CZ) Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE)

How to cite

APA:

Tragardh, J., Pikalek, T., Sery, M., Akimov, D., Meyer, T., Popp, J., & Cizmar, T. (2020). Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope. In Jurgen Popp, Csilla Gergely (Eds.), Proceedings of SPIE - The International Society for Optical Engineering. Virtual, Online, FRA: SPIE.

MLA:

Tragardh, Johanna, et al. "Coherent anti-Stokes Raman scattering microscopy through a multimode fiber endoscope." Proceedings of the Biomedical Spectroscopy, Microscopy, and Imaging 2020, Virtual, Online, FRA Ed. Jurgen Popp, Csilla Gergely, SPIE, 2020.

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