Differential response of liver sinusoidal endothelial cells and hepatocytes to oleic and palmitic acid revealed by Raman and CARS imaging

Matuszyk E, Sierka E, Rodewald M, Bae H, Meyer T, Kus E, Chlopicki S, Schmitt M, Popp J, Baranska M (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Biochimica et Biophysica Acta - Molecular Basis of Disease Elsevier

Book Volume: 1866

Article Number: 165763

Journal Issue: 6

DOI: 10.1016/j.bbadis.2020.165763

Abstract

Excess circulating fatty acids contribute to endothelial dysfunction that subsequently aggravates the metabolic conditions such as fatty liver diseases. However, the exact mechanism of this event is not fully understood, and the investigation on the effect of a direct exposure to fatty acids together with their subsequent fate is of interest. In this work we employed a chemically specific and label-free techniques such as Raman and CARS microscopies, to investigate the process of lipid droplets (LDs) formation in endothelial cells and hepatocytes after exposure to oleic and palmitic acid. We aimed to observe the changes in the composition of LDs associated with metabolism and degradation of lipids. We were able to characterize the diversity in the formation of LDs in endothelium as compared to hepatocytes, as well as the differences in the formation of LDs and degradation manner with respect to the used fatty acid. Thus, for the first time the spectral characteristics of LDs formed in endothelial cells after incubation with oleic and palmitic acid is presented, including the time-dependent changes in their chemical composition.

Involved external institutions

Friedrich-Schiller-Universität Jena DE Germany (DE) Jagiellonian University / Uniwersytet Jagielloński (UJ) PL Poland (PL)

How to cite

APA:

Matuszyk, E., Sierka, E., Rodewald, M., Bae, H., Meyer, T., Kus, E.,... Baranska, M. (2020). Differential response of liver sinusoidal endothelial cells and hepatocytes to oleic and palmitic acid revealed by Raman and CARS imaging. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1866(6). https://doi.org/10.1016/j.bbadis.2020.165763

MLA:

Matuszyk, Ewelina, et al. "Differential response of liver sinusoidal endothelial cells and hepatocytes to oleic and palmitic acid revealed by Raman and CARS imaging." Biochimica et Biophysica Acta - Molecular Basis of Disease 1866.6 (2020).

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