Automated and rapid identification of multidrug resistant Escherichia coli against the lead drugs of acylureidopenicillins, cephalosporins, and fluoroquinolones using specific Raman marker bands

Goetz T, Dahmsz M, Kirchhoff J, Beleites C, Glaser U, Bohnert JA, Pletz MW, Popp J, Schlattmann P, Neugebauer U (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Journal of Biophotonics Wiley-VCH Verlag

Book Volume: 13

Article Number: e202000149

Journal Issue: 8

DOI: 10.1002/jbio.202000149

Abstract

A Raman-based, strain-independent, semi-automated method is presented that allows the rapid (<3 hours) determination of antibiotic susceptibility of bacterial pathogens isolated from clinical samples. Applying a priori knowledge about the mode of action of the respective antibiotic, we identified characteristic Raman marker bands in the spectrum and calculated batch-wise weighted sum scores from standardized Raman intensity differences between spectra of antibiotic exposed and nonexposed samples of the same strains. The lead substances for three relevant antibiotic classes (fluoroquinolone ciprofloxacin, third-generation cephalosporin cefotaxime, ureidopenicillin piperacillin) against multidrug-resistant Gram-negative bacteria (MRGN) revealed a high sensitivity and specificity for the susceptibility testing of two Escherichia coli laboratory strains and 12 clinical isolates. The method benefits from the parallel incubation of control and treated samples, which reduces the variance due to alterations in cultivation conditions and the standardization of differences between batches leading to long-term comparability of Raman measurements.

Involved external institutions

Universitätsklinikum Jena DE Germany (DE) Leibniz-Institut für Photonische Technologien e.V. DE Germany (DE) Chemometrix GmbH DE Germany (DE)

How to cite

APA:

Goetz, T., Dahmsz, M., Kirchhoff, J., Beleites, C., Glaser, U., Bohnert, J.A.,... Neugebauer, U. (2020). Automated and rapid identification of multidrug resistant Escherichia coli against the lead drugs of acylureidopenicillins, cephalosporins, and fluoroquinolones using specific Raman marker bands. Journal of Biophotonics, 13(8). https://doi.org/10.1002/jbio.202000149

MLA:

Goetz, Theresa, et al. "Automated and rapid identification of multidrug resistant Escherichia coli against the lead drugs of acylureidopenicillins, cephalosporins, and fluoroquinolones using specific Raman marker bands." Journal of Biophotonics 13.8 (2020).

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