Vibrational spectroscopic investigation of blood plasma and serum by drop coating deposition for clinical application
Huang J, Ali N, Quansah E, Guo S, Noutsias M, Meyer-Zedler T, Bocklitz T, Popp J, Neugebauer U, Ramoji A (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 22
Pages Range: 1-19
Article Number: 2191
Journal Issue: 4
DOI: 10.3390/ijms22042191
Abstract
In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inho-mogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abun-dant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spec-troscopy.
Involved external institutions
Germany (DE)How to cite
APA:
Huang, J., Ali, N., Quansah, E., Guo, S., Noutsias, M., Meyer-Zedler, T.,... Ramoji, A. (2021). Vibrational spectroscopic investigation of blood plasma and serum by drop coating deposition for clinical application. International Journal of Molecular Sciences, 22(4), 1-19. https://doi.org/10.3390/ijms22042191
MLA:
Huang, Jing, et al. "Vibrational spectroscopic investigation of blood plasma and serum by drop coating deposition for clinical application." International Journal of Molecular Sciences 22.4 (2021): 1-19.
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