Low-cost colorimetric diagnostic screening assay for methicillin resistant Staphylococcus aureus
Raji MA, Chinnappan R, Shibl A, Suaifan G, Weber K, Cialla-May D, Popp J, El Shorbagy E, Al-Kattan K, Zourob M (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 225
Article Number: 121946
DOI: 10.1016/j.talanta.2020.121946
Abstract
The timely diagnosis of MRSA in clinical samples helps to reduce the attendant morbidity/mortality associated with infection due to the organism. The early institution of appropriate therapy or deployment of infection control protocols are dependent on a timely report from the microbiology laboratory. Various assays currently used in the identification of MRSA are associated with inherent shortcomings, thus there is a need to explore newer diagnostic frontiers that can eliminate some of these short comings at a relatively cheap, timely, specific and sensitive manner. We present in this study a MRSA specific optical immunosensor to detect the presence of the pathogen on contaminated surface using control and patient strains. Results revealed a detection limits of 103 CFU mL-1 upon visual observation, and 29 CFU mL-1 as determined by the linear regression equation, following the use of ImageJ to quantify activated cotton swab color intensity. The specificity of the sensor was examined by blind testing a panel of non-MRSA bacteria (E. coli, S. aureus and S. epidermis). Negative visual read-out was observed for all tested non-specific bacteria except for MRSA. Assay takes an average of 5 min and presents a powerful point-of-care diagnostic platform for the detection of MRSA.
Involved external institutions
Friedrich-Schiller-Universität Jena
Germany (DE)
Alfaisal University
Saudi Arabia (SA)
University of Jordan
Jordan (JO)
Germany (DE)
Alfaisal University
Saudi Arabia (SA)
University of Jordan
Jordan (JO)
How to cite
APA:
Raji, M.A., Chinnappan, R., Shibl, A., Suaifan, G., Weber, K., Cialla-May, D.,... Zourob, M. (2021). Low-cost colorimetric diagnostic screening assay for methicillin resistant Staphylococcus aureus. Talanta, 225. https://doi.org/10.1016/j.talanta.2020.121946
MLA:
Raji, Muhabat Adeola, et al. "Low-cost colorimetric diagnostic screening assay for methicillin resistant Staphylococcus aureus." Talanta 225 (2021).
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