Comparison of conventional and shifted excitation Raman difference spectroscopy for bacterial identification

Lorenz B, Guo S, Raab C, Leisching P, Bocklitz T, Roesch P, Popp J (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Journal of Raman Spectroscopy

Book Volume: 53

Pages Range: 1285-1292

Journal Issue: 7

DOI: 10.1002/jrs.6360

Abstract

Raman spectroscopy is an emerging tool for fast bacterial identification. However, Raman spectroscopy is depending on suitable preprocessing of the spectra, thereby background removal is a decisive step for conventional Raman spectroscopy. The background has to be estimated, which is challenging especially for high fluorescence backgrounds. Shifted excitation Raman difference spectroscopy (SERDS) eliminates the background through the experimental procedure and holds as promising approach for highly fluorescent samples. Bacterial Raman spectra might be especially complex because these spectra consist of a multitude of overlapping Raman bands from a large multiplicity of biomolecules and only subtitle differences between the species Raman spectra enable the bacterial identification. Here, we investigate the benefits of SERDS compared with conventional Raman spectroscopy specific for the study and identification of bacteria. The comparison utilizes spectra sets of four bacterial species measured with conventional Raman spectroscopy and SERDS and covers three processing approaches for SERDS spectra, for example, the reconstruction with a non-negative least square algorithm.

Involved external institutions

Friedrich-Schiller-Universität Jena DE Germany (DE) Hochschule Darmstadt (h_da) DE Germany (DE) TOPTICA Photonics AG DE Germany (DE)

How to cite

APA:

Lorenz, B., Guo, S., Raab, C., Leisching, P., Bocklitz, T., Roesch, P., & Popp, J. (2022). Comparison of conventional and shifted excitation Raman difference spectroscopy for bacterial identification. Journal of Raman Spectroscopy, 53(7), 1285-1292. https://doi.org/10.1002/jrs.6360

MLA:

Lorenz, Bjoern, et al. "Comparison of conventional and shifted excitation Raman difference spectroscopy for bacterial identification." Journal of Raman Spectroscopy 53.7 (2022): 1285-1292.

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