Optical clearing of murine bones to study megakaryocytes in intact bone marrow using light-sheet fluorescence microscopy

Gorelashvili MG, Heinze KG, Stegner D (2018)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2018

Journal

Methods in Molecular Biology Humana Press

Publisher: Humana Press Inc.

Series: Methods in Molecular Biology

Book Volume: 1812

Pages Range: 233-253

DOI: 10.1007/978-1-4939-8585-2_14

Abstract

In mammals, the differentiation and maturation of megakaryocytes (MKs) occurs in the bone marrow (BM). The three-dimensional environment influences megakaryopoiesis and platelet release. Thus, imaging MKs within the intact BM is important to understand megakaryopoiesis. Here, we present an optical clearing protocol for intact bones and the subsequent microscopic analysis including image processing to quantitatively assess MK size and distribution. This technique overcomes the limitations of classical sectioning methods as the entire bone can be imaged.

Involved external institutions

Universitätsklinikum Würzburg DE Germany (DE) Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Gorelashvili, M.G., Heinze, K.G., & Stegner, D. (2018). Optical clearing of murine bones to study megakaryocytes in intact bone marrow using light-sheet fluorescence microscopy. In (pp. 233-253). Humana Press Inc..

MLA:

Gorelashvili, Maximilian G., Katrin G. Heinze, and David Stegner. "Optical clearing of murine bones to study megakaryocytes in intact bone marrow using light-sheet fluorescence microscopy." Humana Press Inc., 2018. 233-253.

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