Angay O, Friedrich M, Pinnecker J, Hintzsche H, Stopper H, Hempel K, Heinze KG (2018)
Publication Type: Journal article
Publication year: 2018
Book Volume: 93
Pages Range: 305-313
Journal Issue: 3
DOI: 10.1002/cyto.a.23123
The spleen selectively removes cells with intracellular inclusions, for example, detached nuclear fragments in circulating erythrocytes, called Howell–Jolly Bodies (HJBs). With absent or deficient splenic function HJBs appear in the peripheral blood and can be used as a simple and non-invasive risk-indicator for fulminant potentially life-threatening infection after spleenectomy. However, it is still under debate whether counting of the rare HJBs is a reliable measure of splenic function. Investigating HJBs in premature erythrocytes from patients during radioiodine therapy gives about 10 thousand times higher HJB counts than in blood smears. However, we show that there is still the risk of false-positive results by unspecific nuclear remnants in the prepared samples that do not originate from HJBs, but from cell debris residing above or below the cell. Therefore, we present a method to improve accuracy of image-based tests that can be performed even in non-specialized medical institutions. We show how to selectively label HJB-like clusters in human blood samples and how to only count those that are undoubtedly inside the cell. We found a “critical distance” d
APA:
Angay, O., Friedrich, M., Pinnecker, J., Hintzsche, H., Stopper, H., Hempel, K., & Heinze, K.G. (2018). Image-based modeling and scoring of Howell–Jolly Bodies in human erythrocytes. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 93(3), 305-313. https://doi.org/10.1002/cyto.a.23123
MLA:
Angay, Oguzhan, et al. "Image-based modeling and scoring of Howell–Jolly Bodies in human erythrocytes." Cytometry. Part A : the journal of the International Society for Analytical Cytology 93.3 (2018): 305-313.
BibTeX: Download