Sharpening emitter localization in front of a tuned mirror

Heil HS, Schreiber B, Goetz R, Emmerling M, Dabauvalle MC, Krohne G, Hoefling S, Kamp M, Sauer M, Heinze KG (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Light: Science & Applications CIOMP

Book Volume: 7

Article Number: 99

Journal Issue: 1

DOI: 10.1038/s41377-018-0104-z

Abstract

Single-molecule localization microscopy (SMLM) aims for maximized precision and a high signal-to-noise ratio1. Both features can be provided by placing the emitter in front of a metal-dielectric nanocoating that acts as a tuned mirror2–4. Here, we demonstrate that a higher photon yield at a lower background on biocompatible metal-dielectric nanocoatings substantially improves SMLM performance and increases the localization precision by up to a factor of two. The resolution improvement relies solely on easy-to-fabricate nanocoatings on standard glass coverslips and is spectrally and spatially tunable by the layer design and wavelength, as experimentally demonstrated for dual-color SMLM in cells.

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Heil, H.S., Schreiber, B., Goetz, R., Emmerling, M., Dabauvalle, M.-C., Krohne, G.,... Heinze, K.G. (2018). Sharpening emitter localization in front of a tuned mirror. Light: Science & Applications, 7(1). https://doi.org/10.1038/s41377-018-0104-z

MLA:

Heil, Hannah S., et al. "Sharpening emitter localization in front of a tuned mirror." Light: Science & Applications 7.1 (2018).

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