Fehlhaber B, Heinemann AS, Ruebensam K, Willers M, Voellger L, Pfeifer S, Von Koeckritz-Blickwede M, Viemann D (2019)
Publication Type: Journal article
Publication year: 2019
Book Volume: 9
Article Number: 5919
Journal Issue: 1
DOI: 10.1038/s41598-019-42414-4
Neonatal animal models are increasingly employed in order to unravel age-specific disease mechanisms. Appropriate tools objectifying the clinical condition of murine neonates are lacking. In this study, we tested a scoring system specifically designed for newborn mice that relies on clinical observation and examination. Both, in a neonatal sepsis model and an endotoxic shock model, the scoring results strongly correlated with disease-induced death rates. Full as well as observation-restricted scoring, reliably predicted fatality and the remaining time until death. Clinical scores even proved as more sensitive biomarker than 6 traditionally used plasma cytokine levels in detecting sepsis at an early disease stage. In conclusion, we propose a simple scoring system that detects health impairments of newborn mice in a non-invasive longitudinal and highly sensitive manner. Its usage will help to meet animal welfare requirements and might improve the understanding of neonatal disease mechanisms.
APA:
Fehlhaber, B., Heinemann, A.S., Ruebensam, K., Willers, M., Voellger, L., Pfeifer, S.,... Viemann, D. (2019). A sensitive scoring system for the longitudinal clinical evaluation and prediction of lethal disease outcomes in newborn mice. Scientific Reports, 9(1). https://doi.org/10.1038/s41598-019-42414-4
MLA:
Fehlhaber, Beate, et al. "A sensitive scoring system for the longitudinal clinical evaluation and prediction of lethal disease outcomes in newborn mice." Scientific Reports 9.1 (2019).
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