Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging

Lohoefer F, Buchholz R, Glinzer A, Huber K, Haas H, Kaissis G, Feuchtinger A, Aichler M, Sporns PB, Hoeltke C, Stoelting M, Schilling F, Botnar RM, Kimm MA, Faber C, Walch AK, Zernecke A, Karst U, Wildgruber M (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 10

Article Number: 79

Journal Issue: 1

DOI: 10.1038/s41598-019-57075-6

Abstract

Molecular imaging of atherosclerosis by Magnetic Resonance Imaging (MRI) has been impaired by a lack of validation of the specific substrate responsible for the molecular imaging signal. We therefore aimed to investigate the additive value of mass spectrometry imaging (MSI) of atherosclerosis-affine Gadofluorine P for molecular MRI of atherosclerotic plaques. Atherosclerotic Ldlr−/− mice were investigated by high-field MRI (7 T) at different time points following injection of atherosclerosis-affine Gadofluorine P as well as at different stages of atherosclerosis formation (4, 8, 16 and 20 weeks of HFD). At each imaging time point mice were immediately sacrificed after imaging and aortas were excised for mass spectrometry imaging: Matrix Assisted Laser Desorption Ionization (MALDI) Imaging and Laser Ablation – Inductively Coupled Plasma – Mass Spectrometry (LA-ICP-MS) imaging. Mass spectrometry imaging allowed to visualize the localization and measure the concentration of the MR imaging probe Gadofluorine P in plaque tissue ex vivo with high spatial resolution and thus adds novel and more target specific information to molecular MR imaging of atherosclerosis.

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) Westfälische Wilhelms-Universität (WWU) Münster DE Germany (DE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) King’s College London GB United Kingdom (GB) Universitätsklinikum Würzburg DE Germany (DE)

How to cite

APA:

Lohoefer, F., Buchholz, R., Glinzer, A., Huber, K., Haas, H., Kaissis, G.,... Wildgruber, M. (2020). Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-019-57075-6

MLA:

Lohoefer, Fabian, et al. "Mass Spectrometry Imaging of atherosclerosis-affine Gadofluorine following Magnetic Resonance Imaging." Scientific Reports 10.1 (2020).

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