Heat‐inactivation of human serum destroys c1 inhibitor, pro‐motes immune complex formation, and improves human t cell function
Fante MA, Decking SM, Bruss C, Schreml S, Siska PJ, Kreutz M, Renner K (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 22
Pages Range: 1-17
Article Number: 2646
Journal Issue: 5
DOI: 10.3390/ijms22052646
Abstract
Heat‐inactivation of sera is used to reduce possible disturbing effects of complement factors in cell‐culture experiments, but it is controversially discussed whether this procedure is appro-priate or could be neglected. Here, we report a strong impact of heat‐inactivation of human sera on the activation and effector functions of human CD4+ T cells. While T cells cultured with native sera were characterized by a higher proliferation rate and higher expression of CD28, heat‐inactivated sera shaped T cells towards on‐blast formation, higher cytokine secretion (interferon γ, tumor necrosis factor, and interleukin‐17), stronger CD69 and PD‐1 expression, and increased metabolic ac-tivity. Heat‐inactivated sera contained reduced amounts of complement factors and regulators like C1 inhibitor, but increased concentrations of circulating immune complexes. Substitution of C1 in-hibitor reduced the beneficial effect of heat‐inactivation in terms of cytokine release, whereas sur-face‐molecule expression was affected by the addition of complex forming anti‐C1q antibody. Our data clearly demonstrate a beneficial effect of heat‐inactivation of human sera for T cell experiments but indicate that beside complement regulators and immune complexes other components might be relevant. Beyond that, this study further underpins the strong impact of the complement system on T cell function.
Involved external institutions
Germany (DE)How to cite
APA:
Fante, M.A., Decking, S.-M., Bruss, C., Schreml, S., Siska, P.J., Kreutz, M., & Renner, K. (2021). Heat‐inactivation of human serum destroys c1 inhibitor, pro‐motes immune complex formation, and improves human t cell function. International Journal of Molecular Sciences, 22(5), 1-17. https://doi.org/10.3390/ijms22052646
MLA:
Fante, Matthias A., et al. "Heat‐inactivation of human serum destroys c1 inhibitor, pro‐motes immune complex formation, and improves human t cell function." International Journal of Molecular Sciences 22.5 (2021): 1-17.
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