A Versatile Synthetic Affinity Probe Reveals Inhibitory Synapse Ultrastructure and Brain Connectivity**
Khayenko V, Schulte C, Reis SL, Avraham O, Schietroma C, Worschech R, Nordblom NF, Kachler S, Villmann C, Heinze KG, Schlosser A, Schueler-Furman O, Tovote P, Specht CG, Maric HM (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 61
Article Number: e202202078
Journal Issue: 30
Abstract
Visualization of inhibitory synapses requires protocol tailoring for different sample types and imaging techniques, and usually relies on genetic manipulation or the use of antibodies that underperform in tissue immunofluorescence. Starting from an endogenous ligand of gephyrin, a universal marker of the inhibitory synapse, we developed a short peptidic binder and dimerized it, significantly increasing affinity and selectivity. We further tailored fluorophores to the binder, yielding “Sylite”—a probe with outstanding signal-to-background ratio that outperforms antibodies in tissue staining with rapid and efficient penetration, mitigation of staining artifacts, and simplified handling. In super-resolution microscopy Sylite precisely localizes the inhibitory synapse and enables nanoscale measurements. Sylite profiles inhibitory inputs and synapse sizes of excitatory and inhibitory neurons in the midbrain and combined with complimentary tracing techniques reveals the synaptic connectivity.
Involved external institutions
Julius-Maximilians-Universität Würzburg
Germany (DE)
Universitätsklinikum Heidelberg
Germany (DE)
Hebrew University of Jerusalem
Israel (IL)
Abbelight
France (FR)
École Polytechnique - Université Paris-Saclay
France (FR)
Germany (DE)
Universitätsklinikum Heidelberg
Germany (DE)
Hebrew University of Jerusalem
Israel (IL)
Abbelight
France (FR)
École Polytechnique - Université Paris-Saclay
France (FR)
How to cite
APA:
Khayenko, V., Schulte, C., Reis, S.L., Avraham, O., Schietroma, C., Worschech, R.,... Maric, H.M. (2022). A Versatile Synthetic Affinity Probe Reveals Inhibitory Synapse Ultrastructure and Brain Connectivity**. Angewandte Chemie International Edition, 61(30). https://doi.org/10.1002/anie.202202078
MLA:
Khayenko, Vladimir, et al. "A Versatile Synthetic Affinity Probe Reveals Inhibitory Synapse Ultrastructure and Brain Connectivity**." Angewandte Chemie International Edition 61.30 (2022).
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