Conventional NK Cells and Type 1 Innate Lymphoid Cells Do Not Influence Pathogenesis of Experimental Glomerulonephritis

Rickassel C, Gnirck AC, Shaikh N, Adamiak V, Waterhölter A, Tanriver Y, Neumann K, Huber TB, Gasteiger G, Panzer U, Turner JE (2022)

Publication Type: Journal article

Publication year: 2022

Journal

Journal of Immunology American Association of Immunologists

Book Volume: 208

Pages Range: 1585-1594

Journal Issue: 7

DOI: 10.4049/jimmunol.2101012

Abstract

Innate lymphoid cells (ILCs) that express NK cell receptors (NCRs) and the transcription factor T-bet populate nonlymphoid tissues and are crucial in immune responses against viral infections and malignancies. Recent studies highlighted the heterogeneity of this ILC population and extended their functional spectrum to include important roles in tissue homeostasis and autoimmunity. In this article, we provide detailed profiling of NCR⁺T-bet⁺ ILC populations in the murine kidney, identifying conventional NK (cNK) cells and type 1 ILCs (ILC1s) as the two major subsets. Induction of renal inflammation in a mouse model of glomerulonephritis did not substantially influence abundance or phenotype of cNK cells or ILC1s in the kidney. For functional analyses in this model, widely used depletion strategies for total NCR⁺ ILCs (anti-NK1.1 Ab application) and cNK cells (antiasialoGM1 serum application) were unreliable tools, because they were accompanied by significant off-target depletion of kidney NKT cells and CD8⁺ T cells, respectively. However, neither depletion of cNK cells and ILC1s in NKT cell-deficient mice nor specific genetic deletion of cNK cells in Ncr1^Cre/wt 3 Eomes^fl/fl mice altered the clinical course of experimental glomerulonephritis. In summary, we show in this article that cNK cells and ILC1s are dispensable for initiation and progression of immune-mediated glomerular disease and advise caution in the use of standard Ab depletion methods to study NCR⁺ ILC function in mouse models. The Journal of Immunology, 2022, 208: 1585-1594.

Involved external institutions

Universitätsklinikum Hamburg-Eppendorf (UKE)

Germany (DE) Universitätsklinikum Freiburg

Germany (DE) Julius-Maximilians-Universität Würzburg

Germany (DE)

How to cite

APA:

Rickassel, C., Gnirck, A.C., Shaikh, N., Adamiak, V., Waterhölter, A., Tanriver, Y.,... Turner, J.E. (2022). Conventional NK Cells and Type 1 Innate Lymphoid Cells Do Not Influence Pathogenesis of Experimental Glomerulonephritis. Journal of Immunology, 208(7), 1585-1594. https://doi.org/10.4049/jimmunol.2101012

MLA:

Rickassel, Constantin, et al. "Conventional NK Cells and Type 1 Innate Lymphoid Cells Do Not Influence Pathogenesis of Experimental Glomerulonephritis." Journal of Immunology 208.7 (2022): 1585-1594.

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