A multifunctional mouse model to study the role of Samd3
Peters AE, Knoepper K, Grafen A, Kastenmueller W (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 52
Pages Range: 328-337
Journal Issue: 2
Abstract
The capacity to develop immunological memory is a hallmark of the adaptive immune system. To investigate the role of Samd3 for cellular immune responses and memory development, we generated a conditional knock-out mouse including a fluorescent reporter and a huDTR cassette for conditional depletion of Samd3-expressing cells. Samd3 expression was observed in NK cells and CD8 T cells, which are known for their specific function against intracellular pathogens like viruses. After acute viral infections, Samd3 expression was enriched within memory precursor cells and the frequency of Samd3-expressing cells increased during the progression into the memory phase. Similarly, during chronic viral infections, Samd3 expression was predominantly detected within precursors of exhausted CD8 T cells that are critical for viral control. At the functional level however, Samd3-deficient CD8 T cells were not compromised in the context of acute infection with Vaccinia virus or chronic infection with Lymphocytic choriomeningitis virus. Taken together, we describe a novel multifunctional mouse model to study the role of Samd3 and Samd3-expressing cells. We found that Samd3 is specifically expressed in NK cells, memory CD8 T cells, and precursor exhausted T cells during viral infections, while the molecular function of this enigmatic gene remains further unresolved.
Involved external institutions
Germany (DE)How to cite
APA:
Peters, A.E., Knoepper, K., Grafen, A., & Kastenmueller, W. (2022). A multifunctional mouse model to study the role of Samd3. European Journal of Immunology, 52(2), 328-337. https://doi.org/10.1002/eji.202149469
MLA:
Peters, Annika E., et al. "A multifunctional mouse model to study the role of Samd3." European Journal of Immunology 52.2 (2022): 328-337.
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