Studying complexes between PPI dendrimers and mant-ATP

Szulc A, Appelhans D, Voit B, Bryszewska M, Klajnert B (2013)

Publication Type: Journal article

Publication year: 2013

Journal

Journal of Fluorescence Springer Verlag (Germany)

Book Volume: 23

Pages Range: 349-356

Journal Issue: 2

DOI: 10.1007/s10895-012-1153-y

Abstract

The aim of this study was to investigate the interactions between poly(propylene imine) (PPI) dendrimers and 2′-/3′-O-(N′- methylanthraniloyl)-ATP (Mant-ATP). Mant-ATP was used as a model molecule. Purine and pyrimidine nucleoside analogues are antimetabolites commonly used in therapy for cancer. Drug molecules can complex with dendrimers in two ways: therapeutic agents may be attached in dendrimer interior or bind to functional groups on the surface. Drugs attached to nanoparticles are characterized by improved solubility, pharmacokinetics and stability. Here, we have used poly(propylene imine) dendrimers of the 4th and 5th generations (PPI G4 and PPI G5) with primary amino surface groups partially modified with maltose (PPI-m) or without modification (PPI). We assessed the efficiency of complex formation in relation to dendrimer generation, pH of solution and the type of dendrimer used. A double fluorimetric titration method was used to estimate the binding constant (K b ) and the number of binding centers per molecule of the binding agent (n). © 2013 Springer Science+Business Media New York.

Involved external institutions

University of Lodz / Uniwersytet Łódzki

Poland (PL) Leibniz-Institut für Polymerforschung Dresden e. V.

Germany (DE)

How to cite

APA:

Szulc, A., Appelhans, D., Voit, B., Bryszewska, M., & Klajnert, B. (2013). Studying complexes between PPI dendrimers and mant-ATP. Journal of Fluorescence, 23(2), 349-356. https://dx.doi.org/10.1007/s10895-012-1153-y

MLA:

Szulc, A., et al. "Studying complexes between PPI dendrimers and mant-ATP." Journal of Fluorescence 23.2 (2013): 349-356.

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