Biohybrid structures consisting of biotinylated glycodendrimers and proteins: Influence of the biotin ligand's number and chemical nature on the biotin-avidin conjugation

Ennen F, Boye S, Lederer A, Cernescu M, Komber H, Brutschy B, Voit B, Appelhans D (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Polymer Chemistry Royal Society of Chemistry

Book Volume: 5

Pages Range: 1323-1339

Journal Issue: 4

DOI: 10.1039/c3py01152f

Abstract

We present the bioconjugation of avidin as a central and/or bridging building block with mono-, bi- and tetravalent biotinylated glycodendrimers to fabricate defined supramolecular nanostructures for future (bio)medical applications. For this purpose mono-, bi- and tetravalent biotinylated glycodendrimers, decorated with short alkyl-linked or long PEG-linked biotin ligands, were synthesized and characterized by NMR, IR and mass spectrometry and HABA displacement assay. Various techniques (UV/Vis, DLS, TEM, LILBID-MS and AF4) were used in order to obtain information about the structural properties of different conjugates of avidin and mono-, bi- and tetravalent biotinylated glycodendrimers. The biotin ligand's spacer length, its chemical structure and the degree of biotin functionalization are essential parameters in the formation of nanostructures with avidin having a controlled composition and size dimension up to 100 nm. Biohybrid structures with avidin as a central unit require monovalent glycodendrimers with PEG-linked biotin, while bi- and tetravalent glycodendrimers with short alkyl-linked biotin ligands are more efficient than their counterparts with longer PEG-biotin ligands in the fabrication of defined biohybrid structures (up to 100 nm) with avidin as a bridging unit. The most dominating key issue, combined with other conjugation issues, is the optimal ligand-receptor stoichiometry to fabricate biohybrid structures with diameter of <20, <30 or up to 100 nm. © The Royal Society of Chemistry.

Involved external institutions

Leibniz-Institut für Polymerforschung Dresden e. V. DE Germany (DE) Goethe-Universität Frankfurt am Main DE Germany (DE)

How to cite

APA:

Ennen, F., Boye, S., Lederer, A., Cernescu, M., Komber, H., Brutschy, B.,... Appelhans, D. (2014). Biohybrid structures consisting of biotinylated glycodendrimers and proteins: Influence of the biotin ligand's number and chemical nature on the biotin-avidin conjugation. Polymer Chemistry, 5(4), 1323-1339. https://dx.doi.org/10.1039/c3py01152f

MLA:

Ennen, Franka, et al. "Biohybrid structures consisting of biotinylated glycodendrimers and proteins: Influence of the biotin ligand's number and chemical nature on the biotin-avidin conjugation." Polymer Chemistry 5.4 (2014): 1323-1339.

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