Dendritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: View on protein separation processes and detection of nanogram-scaled albumin in biological samples
Polikarpov N, Potolytsyna V, Bessonova E, Tripp S, Appelhans D, Voit B, Kartsova L (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 1378
Pages Range: 65-73
DOI: 10.1016/j.chroma.2014.11.051
Abstract
Unique properties of dendritic polymers make them promising candidates for application as additives in various analytical methods. From this point, we investigated the potential use of maltose-modified hyperbranched poly(ethylene imine) (PEI-Mal) as a dynamically or covalently bound coating and a pseudostationary phase in capillary electrophoresis. The EOF mobilities were measured at different pH values (2.2, 8.5, and 10.2) using PEI-Mal in the background electrolyte (BGE) and desirable repeatability of the EOF with % RSD (. n=. 50) ≤3.3 was obtained. The influence of pH, polymer concentration, and density of maltose shell on the separation properties of a model mixture of four proteins (albumin, lysozyme, myoglobin, insulin) were investigated. Applying PEI-Mal as a dynamic coating, improved separation of the protein mixture with a high repeatability was achieved. Applying PEI-Mal as a covalent coating for concentrating proteins in the large volume sample stacking (LVSS) combined with the field-enhanced sample injection (FESI), up to 1320-fold enhancement of sensitivity was achieved. The detection limit of 100-500. ng/ml allowed successful analysis of albumin level both in blood and urine samples without additional preconcentration.
Involved external institutions
Leibniz-Institut für Polymerforschung Dresden e. V.
Germany (DE)
Saint Petersburg State University (SPbU) / Санкт-Петербургский государственный университет (СПбГУ)
Russian Federation (RU)
Germany (DE)
Saint Petersburg State University (SPbU) / Санкт-Петербургский государственный университет (СПбГУ)
Russian Federation (RU)
How to cite
APA:
Polikarpov, N., Potolytsyna, V., Bessonova, E., Tripp, S., Appelhans, D., Voit, B., & Kartsova, L. (2015). Dendritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: View on protein separation processes and detection of nanogram-scaled albumin in biological samples. Journal of Chromatography A, 1378, 65-73. https://dx.doi.org/10.1016/j.chroma.2014.11.051
MLA:
Polikarpov, Nikita, et al. "Dendritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: View on protein separation processes and detection of nanogram-scaled albumin in biological samples." Journal of Chromatography A 1378 (2015): 65-73.
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