Nickel electrodes as a cheap and versatile platform for studying structure and function of immobilized redox proteins

Han XX, Li J, Oener IH, Zhao B, Leimkuehler S, Hildebrandt P, Weidinger IM (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Analytica Chimica Acta Elsevier Masson

Book Volume: 941

Pages Range: 35-40

DOI: 10.1016/j.aca.2016.08.053

Abstract

Practical use of many bioelectronic and bioanalytical devices is limited by the need of expensive materials and time consuming fabrication. Here we demonstrate the use of nickel electrodes as a simple and cheap solid support material for bioelectronic applications. The naturally nanostructured electrodes showed a surprisingly high electromagnetic surface enhancement upon light illumination such that immobilization and electron transfer reactions of the model redox proteins cytochrome b5 (Cyt b5) and cytochrome c (Cyt c) could be followed via surface enhanced resonance Raman spectroscopy. It could be shown that the nickel surface, when used as received, promotes a very efficient binding of the proteins upon preservation of their native structure. The immobilized redox proteins could efficiently exchange electrons with the electrode and could even act as an electron relay between the electrode and solubilized myoglobin. Our results open up new possibility for nickel electrodes as an exceptional good support for bioelectronic devices and biosensors on the one hand and for surface enhanced spectroscopic investigations on the other hand.

Involved external institutions

Jilin University (JLU) / 吉林大学 CN China (CN) Technische Universität Berlin DE Germany (DE) Universität Potsdam DE Germany (DE)

How to cite

APA:

Han, X.X., Li, J., Oener, I.H., Zhao, B., Leimkuehler, S., Hildebrandt, P., & Weidinger, I.M. (2016). Nickel electrodes as a cheap and versatile platform for studying structure and function of immobilized redox proteins. Analytica Chimica Acta, 941, 35-40. https://doi.org/10.1016/j.aca.2016.08.053

MLA:

Han, Xiao Xia, et al. "Nickel electrodes as a cheap and versatile platform for studying structure and function of immobilized redox proteins." Analytica Chimica Acta 941 (2016): 35-40.

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