Schwalb B, Michel M, Zacher B, Fruehauf K, Demel C, Tresch A, Gagneur J, Cramer P (2016)
Publication Type: Journal article
Publication year: 2016
Book Volume: 352
Pages Range: 1225-1228
Journal Issue: 6290
Pervasive transcription of the genome produces both stable and transient RNAs.We developed transient transcriptome sequencing (TT-seq), a protocol that uniformly maps the entire range of RNA-producing units and estimates rates of RNA synthesis and degradation. Application of TT-seq to human K562 cells recovers stable messenger RNAs and long intergenic noncoding RNAs and additionally maps transient enhancer, antisense, and promoter-associated RNAs. TT-seq analysis shows that enhancer RNAs are short-lived and lack U1 motifs and secondary structure.TT-seq also maps transient RNA downstream of polyadenylation sites and uncovers sites of transcription termination; we found, on average, four transcription termination sites, distributed in a windowwith amedianwidth of 3300 base pairs.Termination sites coincidewith a DNA motif associated with pausing of RNA polymerase before its release from the genome.
APA:
Schwalb, B., Michel, M., Zacher, B., Fruehauf, K., Demel, C., Tresch, A.,... Cramer, P. (2016). TT-seq maps the human transient transcriptome. Science, 352(6290), 1225-1228. https://doi.org/10.1126/science.aad9841
MLA:
Schwalb, Bjorn, et al. "TT-seq maps the human transient transcriptome." Science 352.6290 (2016): 1225-1228.
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