Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNA

Wang H, Stehr A, Singh J, Zlatar L, Hartmann A, Evert K, Naschberger E, Von Stillfried S, Boor P, Munoz LE, Knopf J, Stürzl M, Herrmann M (2023)

Publication Type: Journal article

Publication year: 2023

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 24

Article Number: 4101

Journal Issue: 4

DOI: 10.3390/ijms24044101

Abstract

During inflammatory responses, neutrophils enter the sites of attack where they execute various defense mechanisms. They (I) phagocytose microorganisms, (II) degranulate to release cytokines, (III) recruit various immune cells by cell-type specific chemokines, (IV) secrete anti-microbials including lactoferrin, lysozyme, defensins and reactive oxygen species, and (V) release DNA as neutrophil extracellular traps (NETs). The latter originates from mitochondria as well as from decondensed nuclei. This is easily detected in cultured cells by staining of DNA with specific dyes. However, in tissues sections the very high fluorescence signals emitted from the condensed nuclear DNA hamper the detection of the widespread, extranuclear DNA of the NETs. In contrast, when we employ anti-DNA-IgM antibodies, they are unable to penetrate deep into the tightly packed DNA of the nucleus, and we observe a robust signal for the extended DNA patches of the NETs. To validate anti-DNA-IgM, we additionally stained the sections for the NET-markers histone H2B, myeloperoxidase, citrullinated histone H3, and neutrophil elastase. Altogether, we have described a fast one-step procedure for the detection of NETs in tissue sections, which provides new perspectives to characterize neutrophil-associated immune reactions in disease.

Authors with CRIS profile

Han Wang Medizinische Fakultät Antonia Stehr Professur für Molekulare und Experimentelle Chirurgie Jeeshan Singh Department of Medicine 3 – Rheumatology and Immunology Leticija Zlatar Department of Medicine 3 – Rheumatology and Immunology Arndt Hartmann Lehrstuhl für Allgemeine Pathologie und Pathologische Anatomie Elisabeth Naschberger Medizinische Fakultät Luis Munoz Becerra Department of Medicine 3 – Rheumatology and Immunology Jasmin Knopf Department of Medicine 3 – Rheumatology and Immunology Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology

Involved external institutions

Universität Regensburg DE Germany (DE) Universitätsklinikum Aachen (UKA) DE Germany (DE)

How to cite

APA:

Wang, H., Stehr, A., Singh, J., Zlatar, L., Hartmann, A., Evert, K.,... Herrmann, M. (2023). Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNA. International Journal of Molecular Sciences, 24(4). https://doi.org/10.3390/ijms24044101

MLA:

Wang, Han, et al. "Anti-DNA-IgM Favors the Detection of NET-Associated Extracellular DNA." International Journal of Molecular Sciences 24.4 (2023).

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