Andres C, Hasenauer J, Ahn HS, Joseph EK, Isensee J, Theis FJ, Allgoewer F, Levine JD, Dib-Hajj SD, Waxman SG, Hucho T (2013)
Publication Type: Journal article
Publication year: 2013
Book Volume: 154
Pages Range: 2216-2226
Journal Issue: 10
DOI: 10.1016/j.pain.2013.07.005
Growth factors such as nerve growth factor and glial cell line-derived neurotrophic factor are known to induce pain sensitization. However, a plethora of other growth factors is released during inflammation and tissue regeneration, and many of them are essential for wound healing. Which wound-healing factors also alter the sensitivity of nociceptive neurons is not well known. We studied the wound-healing factor, basic fibroblast growth factor (bFGF), for its role in pain sensitization. Reverse transcription polymerase chain reaction showed that the receptor of bFGF, FGFR1, is expressed in lumbar rat dorsal root ganglia (DRG). We demonstrated presence of FGFR1 protein in DRG neurons by a recently introduced quantitative automated immunofluorescent microscopic technique. FGFR1 was expressed in all lumbar DRG neurons as quantified by mixture modeling. Corroborating the mRNA and protein expression data, bFGF induced Erk1/2 phosphorylation in nociceptive neurons, which could be blocked by inhibition of FGF receptors. Furthermore, bFGF activated Erk1/2 in a dose- and time-dependent manner. Using single-cell electrophysiological recordings, we found that bFGF treatment of DRG neurons increased the current-density of Na
APA:
Andres, C., Hasenauer, J., Ahn, H.-S., Joseph, E.K., Isensee, J., Theis, F.J.,... Hucho, T. (2013). Wound-healing growth factor, basic FGF, induces Erk1/2-dependent mechanical hyperalgesia. Pain, 154(10), 2216-2226. https://doi.org/10.1016/j.pain.2013.07.005
MLA:
Andres, Christine, et al. "Wound-healing growth factor, basic FGF, induces Erk1/2-dependent mechanical hyperalgesia." Pain 154.10 (2013): 2216-2226.
BibTeX: Download