Real-time near infrared fluorescence (NIRF) intra-operative imaging in ovarian cancer using an αvβ3-integrin targeted agent

Harlaar NJ, Kelder W, Sarantopoulos A, Bart J, Themelis G, Van Dam GM, Ntziachristos V (2013)

Publication Type: Journal article

Publication year: 2013

Journal

Gynecologic Oncology Elsevier

Book Volume: 128

Pages Range: 590-595

Journal Issue: 3

DOI: 10.1016/j.ygyno.2012.12.011

Abstract

Background In ovarian cancer, optimal cytoreductive surgery is of the utmost importance for long-term survival. The ability to visualize minuscule tumor deposits is important to ensure complete resection of the tumor. The purpose of our study was to estimate the in vivo sensitivity, specificity and diagnostic accuracy of an intra-operative fluorescence imaging system combined with an αvβ3-integrin targeted near-infrared fluorescent probe. Method Tumor bearing mice were injected intravenously with a fluorescent probe targeting αvβ3 integrins. Fluorescent spots and non-fluorescent tissue were identified and resected. Standard histopathology and fluorescence microscopy were used as gold-standard for tumor detection. Results Fifty-eight samples excised with support of intra-operative image-guided surgery were analyzed. The mean target to background ratio was 2.2 (SD 0.5). The calculated sensitivity of the imaging system was 95%, and the specificity was 88% with a diagnostic accuracy of 96.5%. Conclusion Near-infrared image-guided surgery in this model has a high diagnostic accuracy and a fair target to background ratio that supports the development towards clinical translation of αvβ 3-integrin targeted imaging. © 2013 Elsevier Inc.

Involved external institutions

Technische Universität München (TUM)

Germany (DE) University of Groningen / Rijksuniversiteit Groningen

Netherlands (NL)

How to cite

APA:

Harlaar, N.J., Kelder, W., Sarantopoulos, A., Bart, J., Themelis, G., Van Dam, G.M., & Ntziachristos, V. (2013). Real-time near infrared fluorescence (NIRF) intra-operative imaging in ovarian cancer using an αvβ3-integrin targeted agent. Gynecologic Oncology, 128(3), 590-595. https://doi.org/10.1016/j.ygyno.2012.12.011

MLA:

Harlaar, N. J., et al. "Real-time near infrared fluorescence (NIRF) intra-operative imaging in ovarian cancer using an αvβ3-integrin targeted agent." Gynecologic Oncology 128.3 (2013): 590-595.

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