Schlaich F, Brons S, Haberer T, Debus J, Combs SE, Weber KJ (2013)
Publication Type: Journal article
Publication year: 2013
Book Volume: 8
Article Number: 260
Journal Issue: 1
Background: Characterization of combination effects of chemotherapy drugs with carbon ions in comparison to photons in vitro.Methods: The human colon adenocarcinoma cell line WiDr was tested for combinations with camptothecin, cisplatin, gemcitabine and paclitaxel. In addition three other human tumour cell lines (A549: lung, LN-229: glioblastoma, PANC-1: pancreas) were tested for the combination with camptothecin. Cells were irradiated with photon doses of 2, 4, 6 and 8 Gy or carbon ion doses of 0.5, 1, 2 and 3 Gy. Cell survival was assessed using the clonogenic growth assay. Treatment dependent changes in cell cycle distribution (up to 12 hours post-treatment) were measured by FACS analysis after propidium-iodide staining. Apoptosis was monitored for up to 36 hours post-treatment by Nicoletti-assay (with qualitative verification using DAPI staining).Results: All cell lines exhibited the well-known increase of killing efficacy per unit dose of carbon ion exposure, with relative biological efficiencies at 10% survival (RBE
APA:
Schlaich, F., Brons, S., Haberer, T., Debus, J., Combs, S.E., & Weber, K.-J. (2013). Comparison of the effects of photon versus carbon ion irradiation when combined with chemotherapy in vitro. Radiation Oncology, 8(1). https://doi.org/10.1186/1748-717X-8-260
MLA:
Schlaich, Fabian, et al. "Comparison of the effects of photon versus carbon ion irradiation when combined with chemotherapy in vitro." Radiation Oncology 8.1 (2013).
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