Three-dimensional imaging of whole mouse models: Comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging
Tapfer A, Bech M, Zanette I, Symvoulidis P, Stangl S, Multhoff G, Molls M, Ntziachristos V, Pfeiffer F (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Book Volume: 253
Pages Range: 24-30
Journal Issue: 1
DOI: 10.1111/jmi.12094
Abstract
In this study, we compare two evolving techniques for obtaining high-resolution 3D anatomical data of a mouse specimen. On the one hand, we investigate cryotome-based planar epi-illumination imaging (cryo-imaging). On the other hand, we examine X-ray phase-contrast micro-computed tomography (micro-CT) using synchrotron radiation. Cryo-imaging is a technique in which an electron multiplying charge coupled camera takes images of a cryo-frozen specimen during the sectioning process. Subsequent image alignment and virtual stacking result in volumetric data. X-ray phase-contrast imaging is based on the minute refraction of X-rays inside the specimen and features higher soft-tissue contrast than conventional, attenuation-based micro-CT. To explore the potential of both techniques for studying whole mouse disease models, one mouse specimen was imaged using both techniques. Obtained data are compared visually and quantitatively, specifically with regard to the visibility of fine anatomical details. Internal structure of the mouse specimen is visible in great detail with both techniques and the study shows in particular that soft-tissue contrast is strongly enhanced in the X-ray phase images compared to the attenuation-based images. This identifies phase-contrast micro-CT as a powerful tool for the study of small animal disease models. © 2013 The Authors.
Involved external institutions
Technische Universität München (TUM)
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
How to cite
APA:
Tapfer, A., Bech, M., Zanette, I., Symvoulidis, P., Stangl, S., Multhoff, G.,... Pfeiffer, F. (2014). Three-dimensional imaging of whole mouse models: Comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging. Journal of Microscopy, 253(1), 24-30. https://doi.org/10.1111/jmi.12094
MLA:
Tapfer, A., et al. "Three-dimensional imaging of whole mouse models: Comparing nondestructive X-ray phase-contrast micro-CT with cryotome-based planar epi-illumination imaging." Journal of Microscopy 253.1 (2014): 24-30.
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