Near-infrared fluorescence cholangiopancreatoscopy: Initial clinical feasibility results
Glatz J, Garcia-Allende PB, Becker V, Koch M, Meining A, Ntziachristos V (2014)
Publication Type: Journal article
Publication year: 2014
Journal
Book Volume: 79
Pages Range: 664-668
Journal Issue: 4
DOI: 10.1016/j.gie.2013.10.008
Abstract
Background The recent clinical propagation of targeted fluorescence agents brings a promising alternative in endoscopy by complementing visual disease detection with molecular biomarkers. Objective Development of near-infrared (NIR) fluorescence cholangiopancreatoscopy in real-time and validation of its clinical use. Design Feasibility study. Setting Tertiary referral center at a large university hospital. Patients Patients with pancreatic and biliary diseases. Interventions Routine cholangiopancreatoscopy with additional wide-field NIR fluorescence imaging. Main Outcome Measurements We adapted a miniature cholangioscope for real-time concurrent wide-field color and NIR fluorescence imaging. Illumination is provided through a custom-designed fiber bundle, and the acquired images are relayed via a dichroic beam splitter to 2 charge-coupled devices for simultaneous measurement. We characterize the sensitivity and resolution and demonstrate the clinical feasibility by detecting indocyanine green localization in 2 patients. Results A spatial optical resolution of approximately 50 μm was achieved, and fluorescent dye concentrations of 17.3 nM could be detected. Elevated fluorescence signals were detected during clinical measurements, and biopsy specimens confirmed the presence of malignancy in both patients. Limitations Feasibility study, limited number of patients. Conclusions The results demonstrate that real-time wide-field fluorescence detection in the NIR range is possible in humans by using adapted endoscopes. The feasibility of detecting indocyanine green in the pancreatobiliary ducts is verified, suggesting that cancer screening at a molecular level might play an increasingly important role in the future. © 2014 by the American Society for Gastrointestinal Endoscopy.
Involved external institutions
Technische Universität München (TUM)
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
How to cite
APA:
Glatz, J., Garcia-Allende, P.B., Becker, V., Koch, M., Meining, A., & Ntziachristos, V. (2014). Near-infrared fluorescence cholangiopancreatoscopy: Initial clinical feasibility results. Gastrointestinal Endoscopy, 79(4), 664-668. https://doi.org/10.1016/j.gie.2013.10.008
MLA:
Glatz, Juergen, et al. "Near-infrared fluorescence cholangiopancreatoscopy: Initial clinical feasibility results." Gastrointestinal Endoscopy 79.4 (2014): 664-668.
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