Limited-projection-angle hybrid fluorescence molecular tomography of multiple molecules

Radrich K, Mohajerani P, Bussemer J, Schwaiger M, Beer AJ, Ntziachristos V (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Journal of Biomedical Optics Society of Photo-optical Instrumentation Engineers (SPIE)

Book Volume: 19

Article Number: 046016

Journal Issue: 4

DOI: 10.1117/1.JBO.19.4.046016

Abstract

An advantage of fluorescence methods over other imaging modalities is the ability to concurrently resolve multiple moieties using fluorochromes emitting at different spectral regions. Simultaneous imaging of spectrally separated agents is helpful in interrogating multiple functions or establishing internal controls for accurate measurements. Herein, we investigated multimoiety imaging in the context of a limited-projection-angle hybrid fluorescence molecular tomography (FMT), and x-ray computed tomography implementation and the further registration with positron emission tomography (PET) data. Multichannel FMT systems may image fluorescent probes of varying distribution patterns. Therefore, it is possible that different channels may require different use of priors and regularization parameters. We examined the performance of automatically estimating regularization factors implementing priors, using data-driven regularization specific for limited-projectionangle schemes. We were particularly interested in identifying the implementation variations between hybrid-FMT channels due to probe distribution variation. For this reason, initial validation of the data-driven algorithm on a phantom was followed by imaging different agent distributions in animals, assuming superficial and deep seated activity. We further demonstrate the benefits of combining hybrid FMT with PET to gain multiple readings on the molecular composition of disease. © 2014 The Authors.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Radrich, K., Mohajerani, P., Bussemer, J., Schwaiger, M., Beer, A.J., & Ntziachristos, V. (2014). Limited-projection-angle hybrid fluorescence molecular tomography of multiple molecules. Journal of Biomedical Optics, 19(4). https://doi.org/10.1117/1.JBO.19.4.046016

MLA:

Radrich, Karin, et al. "Limited-projection-angle hybrid fluorescence molecular tomography of multiple molecules." Journal of Biomedical Optics 19.4 (2014).

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