High-resolution raster scan optoacoustic mesoscopy of genetically modified drosophila pupae

Omar M, Gateau J, Ntziachristos V (2014)

Publication Type: Conference contribution

Publication year: 2014

Journal

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Publisher: SPIE

Book Volume: 8943

Conference Proceedings Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Event location: USA

ISBN: 9780819498564

DOI: 10.1117/12.2036185

Abstract

Optoacoutic mesoscopy aims to bridge the gap between optoacoustic microscopy and optoacoustic tomography. We have developed a setup for optoacoustic mesoscopy where we use a high frequency, high numerical aperture spherically focused ultrasound transducer, with a wide bandwidth of 25-125 MHz. The excitation is performed using a diode laser capable of >500 μJ/pulse, 1.8ns pulse width, 1.4 kHz pulse repetition rate, at 515 nm. The system is capable to penetrate more than 5 mm with a resolution of 7 μm axially and 30 μm transversally. Using high-speed stages and scanning the transducer in a quasi-continuous mode, a field of view of 2×2 mm2 is scanned in less than 2 minutes. The system is suitable for imaging biological samples that have a diameter of 1-5 mm; zebrafish, drosophila melanogaster, and thin biological samples such as the mouse ear and mouse extremities. We have used our mesoscopic setup to generate 3- dimensional images of genetically modified drosophila fly, and drosophila pupae expressing GFP from the wings, high resolution images were generated in both cases, in the fly we can see the wings, the legs, the eyes, and the shape of the body. In the pupae the outline of the pupae, the spiracles at both ends and a strong signal corresponding to the location of the future wings are observed. © 2014 SPIE.

Involved external institutions

Technische Universität München (TUM)

Germany (DE) École supérieure de physique et de chimie industrielles de la ville de Paris (ESPCI Paris)

France (FR)

How to cite

APA:

Omar, M., Gateau, J., & Ntziachristos, V. (2014). High-resolution raster scan optoacoustic mesoscopy of genetically modified drosophila pupae. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. USA: SPIE.

MLA:

Omar, Murad, Jerome Gateau, and Vasilis Ntziachristos. "High-resolution raster scan optoacoustic mesoscopy of genetically modified drosophila pupae." Proceedings of the Photons Plus Ultrasound: Imaging and Sensing 2014, USA SPIE, 2014.

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