Serial sectioning and multispectral imaging system for versatile biomedical applications

Symvoulidis P, Cruz Pérez C, Schwaiger M, Ntziachristos V, Westmeyer GG (2014)

Publication Type: Conference contribution

Publication year: 2014

Publisher: Institute of Electrical and Electronics Engineers Inc.

Pages Range: 890-893

Conference Proceedings Title: 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014

Event location: Beijing, CHN

ISBN: 9781467319591

DOI: 10.1109/isbi.2014.6868014

Abstract

Serial sectioning combined with microscopy provides high resolution volumetric data to complement in-vivo imaging modalities and aid ex-vivo diagnostics. We describe the design of a fully-automated cryomicrotome combined with a multispectral reflection and fluorescence imaging system that enables high-throughput analyses of biological specimens with a large field of view and cellular resolution while keeping the manufacturing and running costs low. We show the performance of the system for representative applications in high-resolution volumetric imaging of reporter animals and multispectral tissue analysis. Furthermore, we demonstrate the versatility of the economical imaging system in applications such as in vivo epifluorescence imaging, histology slide scanning, cell counting and gel electrophoresis documentation.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) Technische Universität München (TUM) DE Germany (DE)

How to cite

APA:

Symvoulidis, P., Cruz Pérez, C., Schwaiger, M., Ntziachristos, V., & Westmeyer, G.G. (2014). Serial sectioning and multispectral imaging system for versatile biomedical applications. In 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014 (pp. 890-893). Beijing, CHN: Institute of Electrical and Electronics Engineers Inc..

MLA:

Symvoulidis, P., et al. "Serial sectioning and multispectral imaging system for versatile biomedical applications." Proceedings of the 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014, Beijing, CHN Institute of Electrical and Electronics Engineers Inc., 2014. 890-893.

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