Quantification of regenerative potential in primary human mammary epithelial cells

Linnemann JR, Miura H, Meixner LK, Irmler M, Kloos UJ, Hirschi B, Bartsch HS, Sass S, Beckers J, Theis FJ, Gabka C, Sotlar K, Scheel CH (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Development Company of Biologists

Book Volume: 142

Pages Range: 3239-3251

Journal Issue: 18

DOI: 10.1242/dev.123554

Abstract

We present an organoid regeneration assay in which freshly isolated human mammary epithelial cells are cultured in adherent or floating collagen gels, corresponding to a rigid orcompliantmatrix environment. In both conditions, luminal progenitors form spheres, whereas basal cells generate branched ductal structures. In compliant but not rigid collagen gels, branching ducts form alveoli at their tips, express basal and luminal markers at correct positions, and display contractility, which is required for alveologenesis. Thereby, branched structures generated in compliant collagen gels resemble terminal ductal-lobular units (TDLUs), the functional units of the mammary gland. Using the membrane metallo-endopeptidase CD10 as a surface marker enriches for TDLU formation and reveals the presence of stromal cells within the CD49f^hi/EpCAM− population. In summary, we describe a defined in vitro assay system to quantify cells with regenerative potential and systematically investigate their interaction with the physical environment at distinct steps of morphogenesis.

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich

Germany (DE) Ludwig-Maximilians-Universität (LMU)

Germany (DE)

How to cite

APA:

Linnemann, J.R., Miura, H., Meixner, L.K., Irmler, M., Kloos, U.J., Hirschi, B.,... Scheel, C.H. (2015). Quantification of regenerative potential in primary human mammary epithelial cells. Development, 142(18), 3239-3251. https://doi.org/10.1242/dev.123554

MLA:

Linnemann, Jelena R., et al. "Quantification of regenerative potential in primary human mammary epithelial cells." Development 142.18 (2015): 3239-3251.

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