Network plasticity of pluripotency transcription factors in embryonic stem cells
Filipczyk A, Marr C, Hastreiter S, Feigelman J, Schwarzfischee M, Hoppe PS, Loeffler D, Kokkaliaris KD, Endele M, Schauberger B, Hilsenbeck O, Skylaki S, Hasenauer J, Anastassiadis K, Theis FJ, Schroeder T (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 17
Pages Range: 1235-1246
Journal Issue: 10
DOI: 10.1038/ncb3237
Abstract
Transcription factor (TF) networks are thought to regulate embryonic stem cell (ESC) pluripotency. However, TF expression dynamics and regulatory mechanisms are poorly understood. We use reporter mouse ESC lines allowing non-invasive quantification of Nanog or Oct4 protein levels and continuous long-term single-cell tracking and quantification over many generations to reveal diverse TF protein expression dynamics. For cells with low Nanog expression, we identified two distinct colony types: one re-expressed Nanog in a mosaic pattern, and the other did not re-express Nanog over many generations. Although both expressed pluripotency markers, they exhibited differences in their TF protein correlation networks and differentiation propensities. Sister cell analysis revealed that differences in Nanog levels are not necessarily accompanied by differences in the expression of other pluripotency factors. Thus, regulatory interactions of pluripotency TFs are less stringently implemented in individual self-renewing ESCs than assumed at present.
Involved external institutions
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Technische Universität Dresden
Germany (DE)
Germany (DE)
Technische Universität Dresden
Germany (DE)
How to cite
APA:
Filipczyk, A., Marr, C., Hastreiter, S., Feigelman, J., Schwarzfischee, M., Hoppe, P.S.,... Schroeder, T. (2015). Network plasticity of pluripotency transcription factors in embryonic stem cells. Nature Cell Biology, 17(10), 1235-1246. https://doi.org/10.1038/ncb3237
MLA:
Filipczyk, Adam, et al. "Network plasticity of pluripotency transcription factors in embryonic stem cells." Nature Cell Biology 17.10 (2015): 1235-1246.
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