Silva JMDSE, Zanette I, Noel PB, Cardoso MB, Kimm MA, Pfeiffer F (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 5
Article Number: 14088
DOI: 10.1038/srep14088
Low inherent contrast in soft tissues has been limiting the use of X-ray absorption micro-computed tomography (micro-CT) to access high-resolution structural information of animal organs. The staining agents used in micro-CT to improve the contrast fail in providing high-quality images of whole organs of animals due to diffusion problems of the staining agent into the sample. We demonstrate a staining protocol that incorporates a biochemical conditioning step prior to exposure to the staining agent that succeeds in overcoming the diffusion problems, thus quickly providing high-quality micro-CT images of whole organs of mammals. Besides of yielding non-distorted three-dimensional information at the same spatial resolution accessible in histological sections, micro-CT images of whole organs stained by our method enable easy screening of slices along any direction of the volume thus demonstrating new possibilities of structural analysis in biomedical science.
APA:
Silva, J.M.D.S.E., Zanette, I., Noel, P.B., Cardoso, M.B., Kimm, M.A., & Pfeiffer, F. (2015). Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography. Scientific Reports, 5. https://doi.org/10.1038/srep14088
MLA:
Silva, Juliana Martins De S. E, et al. "Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography." Scientific Reports 5 (2015).
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