High-resolution epi-illumination raster-scan optoacoustic mesoscopy for imaging of model organisms and microvessels

Omar M, Soliman D, Gateau J, Ntziachristos V (2015)

Publication Type: Conference contribution

Publication year: 2015

Journal

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Publisher: SPIE

Book Volume: 9539

Conference Proceedings Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Event location: Munich, DEU

ISBN: 9781628417043

DOI: 10.1117/12.2183624

Abstract

We have developed an epi-illumination raster-scan optoacoustic mesoscopy system (RSOM), the new system is capable of imaging model organisms, and vasculature. The newly developed system is based on a custom designed; spherically focused detector with a Characterization of the system shows an isotropic lateral resolution of 18 μm, and an axial resolution of 4 μm. The scan times are on the order of 8 minutes for a field of view of 10×10 mm2. The achieved resolution is slightly degraded up to a depth of 5 mm. After characterizing the system we showcase it's performance on a zebrafish ex vivo, and an excised mouse ear. Additionally, to improve the visibility of small structures we have reconstructed the high frequencies, and the low frequencies separately, and at the end overplayed the two reconstructions using different colors, this way the high frequencies are not masked by the low frequencies which have a higher signal to noise ratio.

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) PSL Research University / Université de recherche Paris Sciences et Lettres FR France (FR)

How to cite

APA:

Omar, M., Soliman, D., Gateau, J., & Ntziachristos, V. (2015). High-resolution epi-illumination raster-scan optoacoustic mesoscopy for imaging of model organisms and microvessels. In Roger Zemp, Vasilis Ntziachristos (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Munich, DEU: SPIE.

MLA:

Omar, Murad, et al. "High-resolution epi-illumination raster-scan optoacoustic mesoscopy for imaging of model organisms and microvessels." Proceedings of the Opto-Acoustic Methods and Applications in Biophotonics II, Munich, DEU Ed. Roger Zemp, Vasilis Ntziachristos, SPIE, 2015.

BibTeX: Download