Hybrid label-free multiphoton and optoacoustic microscopy (MPOM)
Soliman D, Tserevelakis GJ, Omar M, Ntziachristos V (2015)
Publication Type: Conference contribution
Publication year: 2015
Journal
Publisher: SPIE
Book Volume: 9539
Conference Proceedings Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Event location: Munich, DEU
ISBN: 9781628417043
DOI: 10.1117/12.2183619
Abstract
Many biological applications require a simultaneous observation of different anatomical features. However, unless potentially harmful staining of the specimens is employed, individual microscopy techniques do generally not provide multi-contrast capabilities. We present a hybrid microscope integrating optoacoustic microscopy and multiphoton microscopy, including second-harmonic generation, into a single device. This combined multiphoton and optoacoustic microscope (MPOM) offers visualization of a broad range of structures by employing different contrast mechanisms and at the same time enables pure label-free imaging of biological systems. We investigate the relative performance of the two microscopy modalities and demonstrate their multi-contrast abilities through the label-free imaging of a zebrafish larva ex vivo, simultaneously visualizing muscles and pigments. This hybrid microscopy application bears great potential for developmental biology studies, enabling more comprehensive information to be obtained from biological specimens without the necessity of staining.
Involved external institutions
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Foundation for Research and Technology-Hellas (FORTH) / Ίδρυμα Τεχνολογίας και Έρευνας
Greece (GR)
Germany (DE)
Foundation for Research and Technology-Hellas (FORTH) / Ίδρυμα Τεχνολογίας και Έρευνας
Greece (GR)
How to cite
APA:
Soliman, D., Tserevelakis, G.J., Omar, M., & Ntziachristos, V. (2015). Hybrid label-free multiphoton and optoacoustic microscopy (MPOM). In Roger Zemp, Vasilis Ntziachristos (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Munich, DEU: SPIE.
MLA:
Soliman, Dominik, et al. "Hybrid label-free multiphoton and optoacoustic microscopy (MPOM)." Proceedings of the Opto-Acoustic Methods and Applications in Biophotonics II, Munich, DEU Ed. Roger Zemp, Vasilis Ntziachristos, SPIE, 2015.
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