Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent

Kirscher L, Dean-Ben XL, Scadeng M, Zaremba A, Zhang Q, Kober C, Fehm TF, Razansky D, Ntziachristos V, Stritzker J, Szalay AA (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Theranostics Ivyspring International Publisher

Book Volume: 5

Pages Range: 1045-1057

Journal Issue: 10

DOI: 10.7150/thno.12533

Abstract

We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system.

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE) Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE) University of California, San Diego US United States (USA) (US) Genelux US United States (USA) (US)

How to cite

APA:

Kirscher, L., Dean-Ben, X.L., Scadeng, M., Zaremba, A., Zhang, Q., Kober, C.,... Szalay, A.A. (2015). Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent. Theranostics, 5(10), 1045-1057. https://dx.doi.org/10.7150/thno.12533

MLA:

Kirscher, Lorenz, et al. "Doxycycline inducible melanogenic vaccinia virus as theranostic anti-cancer agent." Theranostics 5.10 (2015): 1045-1057.

BibTeX: Download