High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography
Stiel AC, Dean-Ben XL, Jiang Y, Ntziachristos V, Razansky D, Westmeyer GG (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 40
Pages Range: 367-370
Journal Issue: 3
DOI: 10.1364/OL.40.000367
Abstract
Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins' contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically encoded photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high-contrast molecular optoacoustic imaging.
Involved external institutions
Max-Planck-Institut für Entwicklungsbiologie / Max Planck Institute for Developmental Biology
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
Germany (DE)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
How to cite
APA:
Stiel, A.C., Dean-Ben, X.L., Jiang, Y., Ntziachristos, V., Razansky, D., & Westmeyer, G.G. (2015). High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography. Optics Letters, 40(3), 367-370. https://doi.org/10.1364/OL.40.000367
MLA:
Stiel, Andre C., et al. "High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography." Optics Letters 40.3 (2015): 367-370.
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